Human Retrovirus Protocols: Virology and Molecular Biology Tuofu Zhu

ISBN: 9781617375996

Published: November 19th 2010

Paperback

494 pages


Description

Human Retrovirus Protocols: Virology and Molecular Biology  by  Tuofu Zhu

Human Retrovirus Protocols: Virology and Molecular Biology by Tuofu Zhu
November 19th 2010 | Paperback | PDF, EPUB, FB2, DjVu, AUDIO, mp3, ZIP | 494 pages | ISBN: 9781617375996 | 6.77 Mb

A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of humanMoreA cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host.

Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.



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